Education

• 08/2009-12/2016, Ph.D. in Biochemistry, College of Agriculture, Purdue University, West Lafayette, USA. Advisor: Dr. Scott Briggs

• 08/2005-06/2009, B.S. in Biological Science, College of Life science, Peking University, Beijing, China. Advisor: Dr. Xiaofeng Zheng

Research Interests

• Yeast synthetic biology, fungal pathogenicity and drug resistance, vaccine design of companion animals.

Training and Professional Experience

• 2019-now, Associate Professor, China Agricultural University College of Veterinary Medicine

• 2017-2019, Postdoctoral Fellow, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, China. Advisor: Dr. Jens Nielsen

Selected Publications

[1] Zhibo Yang#, Zan Chen#, and Yueping Zhang*, A simple and economical site-directed mutagenesis method for large plasmids by direct transformation of two overlap PCR. Biotechniques. 2022, DOI: 10.2144/btn-2022-0085

[2] Yijin Zhao, Kaiwen Meng, Jinyu Fu, Shijie Xu, Guang Cai, Geng Meng, Jens Nielsen, Zihe Liu* & Yueping Zhang*, Protein engineering of invertase for enhancing yeast dough fermentation under high sucrose conditions. Folia Microbiologica 2022 Oct 6, DOI: 10.1007/s12223-022-01006-y

[3] Liyang Wang#, Jinyu Fu#, Guang Cai, Xiyu Cheng, Di Zhang, Shuobo Shi*, and Yueping Zhang*, Rapid and Visual RPA-Cas12a Fluorescence Assay for Accurate Detection of Dermatophytes in Cats and Dogs. Biosensors (Basel) 2022, 12(8), 636; DOI: 10.3390/bios12080636

[4] Zehui Qu#, Hongtao Kang#, Chenxi Cui, Kaiwen Meng, Xinzheng Zhang, Liandong Qu, Yueping Zhang*, Geng Meng*, Purification-induced damage to calicivirus particles at near-atomic resolution. Journal of General Virology 2022 103(5), DOI: 10.1099/jgv.0.001742

[5] Kaiwen Meng#, Yueping Zhang#, Qi Liu, Yangnan Huyan, Wenzhuang Zhu, Ye Xiang and Geng Meng*, Structural Design and Assessing of Recombinantly Expressed African Swine Fever Virus p72 Trimer in Saccharomyces cerevisiae, Frontiers in Microbiology, 2022, 13:802098, DOI: 10.3389/fmicb.2022.802098

[6] Wenzhuang Zhu, Kaiwen Meng, Yueping Zhang, Zhigao Bu, Dongming Zhao*, Geng Meng*, Lateral Flow Assay for the Detection of African Swine Fever Virus Antibodies Using Gold Nanoparticle-Labeled Acid-Treated p72. Front Chem. 2022 Jan 3;9:804981. DOI: 10.3389/fchem.2021.804981

[7] Kortany M Baker, Smriti Hoda, Debasmita Saha, Justin B Gregor, Livia Georgescu, Nina D Serratore, Yueping Zhang, Lizhi Cheng, Nadia A Lanman, Scott D Briggs, The Set1 Histone H3K4 Methyltransferase Contributes to Azole Susceptibility in a Species-Specific Manner by Differentially Altering the Expression of Drug Efflux Pumps and the Ergosterol Gene Pathway. Antimicrob Agents Chemother. 2022 May 17;66(5):e0225021. DOI: 10.1128/aac.02250-21.

[8] Junhao Cheng, Marhaba Ahmat, Henan Guo, Xubiao Wei, Lulu Zhang, Qiang Cheng, Jing Zhang, Junyong Wang, Dayong Si, Yueping Zhang*, Rijun Zhang*, Expression, Purification and Characterization of a Novel Hybrid Peptide CLP with Excellent Antibacterial Activity. Molecules 2021, 26, 7142. DOI: 10.3390/molecules26237142

[9] Junhao Cheng, Mingkun Wu, Ren Zhong, Dayong Si, Geng Meng, Rijun Zhang*, Yueping Zhang*, Rapid Golden Gate assembly of exons from genomic DNA for protein expression in Escherichia coli and Pichia pastoris. Biotechniques. 2021 Aug 10. DOI: 10.2144/btn-2021-0039

[10] Yu Fu, Zhenglin Zhu, Geng Meng, Rijun Zhang*, Yueping Zhang*, A CRISPR‑Cas9 based shuffle system for endogenous histone H3 and H4 combinatorial mutagenesis. Scientific Reports. 2021, Feb 8;11(1):3298, DOI: 10.1038/s41598-021-82774-4

[11] Jinyu Fu#, Yueping Zhang#, Guang Cai, Geng Meng*, Shuobo Shi*, Rapid and sensitive RPA-Cas12a-fluorescence assay for point-of-care detection of African swine fever virus. PLoS One. 2021 Jul 19;16(7):e0254815. DOI: 10.1371/journal.pone.0254815

[12] Guiping Gong#, Yueping Zhang#, Zibai Wang, Luo Liu, Shuobo Shi, Verena Siewers, Qipeng Yuan, Jens Nielsen, Xu Zhang*, and Zihe Liu*, GTR 2.0: gRNA-tRNA Array and Cas9-NG Based Genome Disruption and Single-Nucleotide Conversion in Saccharomyces cerevisiae. ACS Synthetic Biology. 2021 May 20, DOI: 10.1021/acssynbio.0c00560

[13] Yijin Zhao#, Yueping Zhang#, Jens Nielsen, Zihe Liu*, Production of β‐carotene in Saccharomyces cerevisiae through altering yeast lipid metabolism. Biotechnology and Bioengineering. 2021 May;118(5):2043-2052. DOI: 10.1002/bit.27717

[14] Yueping Zhang#, Juan Wang#, Zibai Wang, Yiming Zhang, Shuobo Shi, Jens Nielsen, Zihe Liu*, A gRNA-tRNA Array CRISPR-Cas9 Based Rapid Multiplexed Genome Editing in Saccharomyces cerevisiae. Nature Communications. 2019 Mar 5;10(1):1053, DOI: 10.1038/s41467-019-09005-3

[15] Zihe Liu, Yueping Zhang, Jens Nielsen*; Synthetic Biology of Yeast. Biochemistry. 2019 Mar 19;58(11):1511-1520, DOI: 10.1021/acs.biochem.8b01236

[16] Yueping Zhang, Jens Nielsen, Zihe Liu*, Engineering yeast metabolism for production of terpenoids for use as perfume ingredients, pharmaceuticals and biofuels. FEMS Yeast Research. 2017 Dec 1;17(8), DOI: 10.1093/femsyr/fox080 

[17] Yueping Zhang, Nina D. Serratore, Scott D. Briggs*, (2017) N-ICE plasmids for generating N-terminal 3×FLAG tagged genes that allow Inducible, Constitutive, or Endogenous expression in Saccharomyces cerevisiae. Yeast. 2017 May;34(5):223-235, DOI: 10.1002/yea.3226

Note: # co-first author, * Corresponding author.