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>> 科学研究 >> 师资队伍 >> 基础兽医系 >> 教授
姓名:王战辉 性别: 职务:
所在系:基础兽医系 职称:研究员 导师类别:博导/硕导
政治面貌:中共党员 出生年月:1979.06 学位:博士
电子邮件:wangzhanhui@cau.edu.cn 办公电话:62734565

王战辉,男,1979年6月出生,博士,研究员,博士生导师, 入选2012年教育部新世纪优秀人才支持计划。主要从事小分子有害化合物(兽药、霉菌毒素、非法添加剂)的抗体制备、快速检测技术及分子识别机制研究。近五年主持参加的科研项目30余项,国内外学术刊物发表研究论文100余篇,SCI收录80余篇,申请国家发明专利30余项,参与制定国家标准10余项。2008年获第一届“北京市优秀博士论文”,2009年获全国百篇优秀博士论文提名奖。

代表性文章:

1. Zhanhui Wang, Tiejun Mi, Huiyan Zhang, Yajie Sheng, Weimin Shi, Suxia Zhang, Jianzhong Shen. Hapten synthesis, monoclonal antibody production, and development of a competitive indirect enzyme-linked immunosorbnent assay for erythromycin in milk. Food Chemistry, 2015, 171, 98-107.

2. Xiao Liang, Hengjia Ni, Ross C. Beier, Yanni Dong, Jingya Li, Xiangshu Luo, Suxia Zhang, Jianzhong Shen, Zhanhui Wang*. Highly broad-specific and sensitive enzyme-linked immunosorbent assay for screening of the sulfonamides: Assay optimization and application to milk samples. Food Analytical Methods, 2014, 7, 1992-2002.

3. Zhanhui Wang, Qi Liang, Jianzhong Shen*. A Novel Ligand for the Affinity-Chromatographic Purification of Monoclonal Antibody to Ractopamine. Journal of Chmatography B, 2014, 971, 10-13.

4. Zhanhui Wang, Heng Li, Chenglong Li, Qing Yu, Jianzhong Shen*, Sarah De Saeger.  Development and application of a quantitative fluorescence-based immunochromatographic assay for fumonisin B1 in maize. Journal of Agricultural Food and Chemistry, 2014, 62(27): 6294–6298.

5. Beloglazova N.V., Speranskaya E.S., Wu A, Zhanhui Wang, Sanders M, Goftman V., Zhang D. Goryacheva I.Yu., De Saeger S. Novel multiplex fluorescent immunoassays based on quantum dot nanolabels for mycotoxins determination . Analytical Chemistry, 2014, 62, 59-65.

6. Tiejun Mi, Suxia Zhang a, Jianzhong Shen, Sergei A. Eremin, Zhanhui Wang*. Development and Optimization of Fluorescence Polarization Immunoassay for Orbifloxacin in Milk. Analytical methods, 2014, 6 (11), 3849 - 3857.

7. Yajie Sheng, Sergei A Eremin,Tiejun Mi, Suxia Zhang, Jianzhong Shen, Zhanhui Wang*. The development of a fluorescence polarization immunoassay for aflatoxin detection. Biomedical and Environmental Sciences, 2014, 27(2): 126-129.

8. Zhanhui Wang, Huiyan Zhang, Hengjia Ni, Suxia Zhang, Jianzhong Shen. Development of a highly sensitive and specific immunoassay for enrofloxacin based on heterologous coating haptens. Analytica Chimica Acta. 2014, 820(11): 152–158.

9. Elena S Speranskaya, Natalia V Beloglazova, Pieterjan Lenain, Sarah De Saeger, Zhanhui Wang, Suxia Zhang, Zeger Hens, Dietmar Knopp, Reinhard Niessner, Dmitry V Potapkin, Irina Yu Goryacheva. Polymer-coated fluorescent CdSe-based quantum dots for application in immunoassay. Biosensors & bioelectronics. 2014, 53(15): 225-231.

10. Wenxiao Jiang, Huiyan Zhang, Xiangmei Li, Xinxin Zhang, Suxia Zhang, Jianzhong, Shen, Zhanhui Wang*. Monoclonal antibody production and the development of an indirect competitive enzyme-linked immunosorbent assay for screening spiramycin in milk. Journal of Agricultural and Food Chemistry, 2013, 61 (46), 10925–10931

11. Rui Chen, Heng Li, Han Zhang, Suxia Zhang,Weimin Shi, Jianzhong Shen, Zhanhui Wang* Development of a lateral flow fluorescent microsphere immunoassay for the determination of sulfamethazine in milk. Analytical Bianalytical Chemistry, 2013, 405(21): 6783-6789.

12. Zhanhui Wang, Yonghan Li,  Xiao Liang,  Suxia Zhang, Weimin Shi, Jianzhong Shen.  Forcing immunoassay for sulfonamides to higher sensitivity and broader detection spectrum by site heterologous hapten inducing affinity improvement. Analytical Methods, 2013, 5, 6990-7000.

13. Xiao Liang, Zhanhui Wang, Chunmei Wang, Kai Wen, Tiejun Mi, Jing Zhang, Suxia Zhan. A proof-of-concept receptor-based assay for sulfonamides. Analytical Biochemistry, 2013,438, 110-116. Co-first author,

14. Zhanhui Wang, Ross C. Beier, Yajie Sheng, Suxia Zhang, Wenxiao Jiang,  Zhaopeng Wang, Jin Wang, Jianzhong Shen. Monoclonal antibodies with group-specificity toward sulfonamides: selection of hapten and antibody selectivity. Analytical Biochemical Chemistry, 2013, 405(12): 4027-4037.

15. Zhanhui Wang, Na Li, Suxia Zhang, Shuangyang Ding, Jianzhong Shen.Production of antibodies and development of enzyme-linked immunosorbent assay for valnemulin in porcine liver. Food Additives and Contaminants. 2013, 30(2):244-252.

16. Jing Zhang, Zhanhui Wang, Kai Wen, Xiao Liang, Jianzhong Shen. Penicillin-binding protein 3 of Streptococcus pneumonia and its application in screening of β-lactams in milk, Analytical Biochemistry, 2013, 442(2), 158-165.

17. Tiejun Mi, Zhanhui Wang, Sergei A. Eremin, Jianzhong Shen, Suxia Zhang*. Simultaneous Determination of Multiple (Fluoro) quinolone Antibiotics in Food Samples by A One-Step Fluorescence Polarization Immunoassay. Journal of Agricultural and Food Chemistry, 2013, 61 (39), 9347–9355.

18. Wenxiao Jiang, Zhanhui Wang; Beier, R. C.; Jiang, H.; Wu, Y.; Shen, J., Simultaneous Determination of 13 Fluoroquinolone and 22 Sulfonamide Residues in Milk by a Dual-Colorimetric Enzyme-Linked Immunosorbent Assay. Anal Chem 2013, 85 (4), 1995-1999.

19. Jia Wang, Zhanhui Wang, Jing Liu, Hao Li, Qing X. Li, Ji Li, Ting Xu. Nanocolloidal gold-based immuno-dip strip assay for rapid detection of Sudan red I in food samples. Food Chemistry, 2013,136: 1478–1483

20. 王战辉,米铁军,沈建忠. 荧光偏振免疫分析检测粮食及其制品中的真菌毒素研究进展. 中国农业科学. 2012, 45(23):4862-4872.

21. Yajie Sheng, Wenxiao Jiang, Sarah De Saeger, Jianzhong Shen, Suxia Zhang, Zhanhui Wang*. Development of a sensitive enzyme-linked immunosorbent assay for the detection of fumonisin B1 in maize. Toxicon. 2012, 60(7):1245–1250

22. Yanshen Li, Weimin Shi, Jianzhong Shen, Suxia Zhang, Linli Cheng and Zhanhui Wang*. Development of a rapid competitive indirect ELISA procedure for the determination of deoxynivalenol in cereals. Food and Agricultural Immunology. 2012, 23(1): 41-49

23. Zhanhui Wang, Zhenpeng Kai, Ross C. Beier, Jianzhong Shen, Xinling Yang . Investigation of Antigen-Antibody Interactions of Sulfonamides with a Monoclonal Antibody in a Fluorescence Polarization Immunoassay Using 3D-QSAR Models. International Journal of Molecular Sciences. International Journal of Molecular Sciences, 2012, 13, 6334-6351.

24. Yanshen Li, Zhanhui Wang, Sarah De Saeger, Weimin Shi, Cun Li, Suxia Zhang, Xingyuan Cao, Jianzhong Shen. Determination of deoxynivalenol in cereals by immunoaffinity clean-up and ultra-high performance liquid chromatography tandem mass spectrometry. Method. 2012, 56(2): 192-197 Co-author

25. Zhanhui Wang, Pengjie Luo, Linli Cheng, Suxia Zhang, Jianzhong Shen. Hapten–antibody recognition studies in competitive immunoassay of a-zearalanol analogs by computational chemistry and Pearson Correlation analysis 2011, Journal of Molecular Recognition. 24(5):815-823.

26. Zhanhui Wang, Jing Zhang, Suxia Zhang, Jianzhong Shen. Heterologous structure of coating antigen on sensitivity of ELISA for sulfamethazine: evidence from molecular similarity analysis. 2011, Food and Agricultural Immunology. 22(2):115-124

27. Zhanhui Wang, Linli Cheng, Weimin Shi, Suxia Zhang, Jianzhong Shen. Fluorescence Polarization Immunoassay for Salinomycin Based on Monoclonal Antibody. 2010. Science in China Series B-Chemistry.53(3):553-555

28. Zhanhui Wang, Xingyuan Cao, Zhenpeng Kai, Ross C. Beier, Congming Wu, Haiyang Jiang, Linli Cheng, Jianzhong Shen*, Xinling Yang. Three Dimensional Quantitative Structure-Activity Relationships of Sulfonamides Binding Monoclonal Antibody by Comparative Molecular Field Analysis. 2008, Nature Precedings (2008)

29. Cun Li*, Zhanhui Wang*, Xingyuan Cao, Ross C. Beier, Suxia Zhang, Shuangyang Ding, Xiaowei Li, and Jianzhong Shen, Development of an Immunoaffinity Column Method Using Broad-Specificity Monoclonal Antibodies for Simultaneous Extraction and Cleanup of Fluoroquinolone and Sulfonamide Antibiotics in Animal Muscle Tissues. 2008, Journal of Chromatography A, 1209(1-2), 1-9.(*co-first author)

30. Zhanhui Wang, Suxia Zhang, Nailya R. Murtazina, Sergei A. Eremin, Jianzhong Shen, Determination of the veterinary drug maduramicin in food by Fluorescence Polarization Immunoassay. 2008, International Journal of Food Science and Technology, 43, 114-122.

31. Zhanhui Wang, Suxia. Zhang, Shuangyang Ding, Seiger. A. Eremin, Jianzhong Shen. Simultaneous determination of sulphamerazine, sulphamethazine and sulphadiazine in honey and chicken muscle by fluorescence polarisation immunoassay based on monoclonal antibody. Food Additives and Contaminants, 2008, 25 (5): 574-582.

32. 王战辉,丁双阳,张素霞, 沈建忠. 分子模拟技术研究17种磺胺类药物与抗体亲和力构效关系. 2008. 化学学报. 66 (23): 2613-2619.

33. 王战辉,张素霞, 沈建忠. 荧光偏振免疫分析检测磺胺甲基嘧啶研究. 2008, 光谱学与光谱分析. 28(7):1621-1625.

34. 王战辉,张素霞,丁双阳,沈建忠. 荧光偏振免疫分析法研究17种磺胺类药物与抗体亲和力的构效关系. 2008, 高等学校化学学报. 29(6): 1107-1111.

35. Zhanhui Wang, Yan Zhu, Shuangyang Ding, Fangyang He, Ross C.Beier, Jiancheng Li, Haiyang Jiang, Caiwei Feng, Yuping Wan, Suxia Zhang, Zhenpeng Kai, Xinling Yang, Jianzhong Shen. Development of a Monoclonal Antibody-Based Broad-Specificity ELISA for Fluoroquinolone Antibiotics in Foods and Molecular Modeling Studies of Cross-Reactive Compounds. 2007, Analytical Chemistry, 79 (12): 4471-4478.

36. Zhanhui Wang, Suxia Zhang, Irina S. Nesterenkob, Sergei A. Ereminb, Jianzhong Shena,Monoclonal Antibody-Based Fluorescence Polarization Immunoassay for Sulfamethoxypyridazine and Sulfachloropyridazine. 2007, Journal of Agriculture and Food Chemistry, 55(17): 6871- 6878.

 

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