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Dissecting the molecular composition and function of the conoid in Toxoplasma gondii

发布日期:2017-12-21访问次数: 信息来源:动物医学院字号:[ ]


    报告人:龙少军 博士

    时  间:201712269:00

    地  点:动医动科大楼418

 

        欢迎大家参加。

    联系人:刘晶 62732622

  

个人简介

200511月至20107月在捷克国家科学院寄生虫学研究所攻读博士学位,研究布氏锥虫线粒体生物学。毕业后获得欧盟玛丽居里博士后学者计划的资助,在英国纽卡斯尔大学医学院从事微孢子虫、贾第虫等寄生原虫中高度退化线粒体的蛋白质组学、细胞和进化生物学。

20136月开始在美国圣路易斯华盛顿大学医学院分子微生物学系David Sibley实验室从事弓形虫的寄生生物学和病原生物学研究。主要研究方向为弓形虫的钙信号及其抑制药物的筛选、遗传操作工具的改造和顶体复合体(apical complex)的分子构成和功能。近2-3年来,对顶体复合体的研究获得了突破性的进展,发现了一系列的新蛋白质,深入探讨了这些蛋白质的功能作用(Long S, et al. Nature Communications, 发表日期:20171221日),并深入研究了定位于该细胞结构的钙调蛋白的功能(Long S et al, PLoS Pathogens, 2017)。改造和开发的新遗传操作技术整合在上述两项研究工作中,发表后得到F1000专家的推荐和点评,并受邀为Bio-Protocol写作章节。

 

发表论文情况

1. Long S, Brown KM, Sibley LD. 2017. Protein interactomes defined by BirA labeling in Toxoplasma gondii. Bio-Protocol. Invited book chapter submitted.

2. Brown KM, Long S, Sibley LD. 2017. Studying essential proteins using auxin induced degron (AID) fusions in Toxoplasma gondii. Bio-Protocol. Invited book chapter submitted.

3. Long S, Anthony B, Drewry L, Sibley LD. 2017. A conserved ankyrin repeat containing protein CPH1 regulates conoid stability, motility and cell invasion in Toxoplasma gondii. Nature Communications. 8:2236(2017). Doi:10.1038/s41467-017-02341-2. 

4. Rutaganira F, Barks J, Dhason M, Wang Q, Lopez M, Long S, Radke J, Jones N, Maddirala A, Janetka J, El Bakkouri M, Hui R, Shokat K, Sibley LD. 2017. Inhibition of calcium dependent protein kinase 1 (CDPK1) by pyrazolopyrimidine analogs decreases establishment and reoccurrence of central nervous system disease by Toxoplasma gondii. J Med Chem. Accepted. doi: 10.1021/acs.jmedchem.

5. Long S, Brown K, Drewry L, Anthony B, Phan, IQ, Sibley LD. 2017. Calmodulin-like proteins localized to the conoid regulate motility and cell invasion by Toxoplasma gondii. PLoS Pathogens. 13(5):e1006379. Faculty 1000 recommendations  Microbiology.

6. Brown K, Long S, Sibley LD. 2017. Plasma membrane-association by N-acylation governs PKG function in Toxoplasma gondii. mBio. 8(3):e00375-17.

7. Molik S, Goldberg AV, Hacker C, Freibert SA, Dean P, Williams TA, Nakjang S, Long S, Sendra K, Heinz E, Hirt RP, Lucocq JM, Embley TM, Lill R. 2017. Evolutionary conservation and in vitro reconstitution of microsporidian iron-sulfur cluster biosynthesis. Nature Communications. 8:13932.

8. Bang S, Brown K, Long S, Sibley LD. 2017. Development of CRISPR/Cas9 for efficient genome editing in Toxoplasma gondii. Methods in Molecular Biology. 1498:79-103. 

9. Long S, Wang Qiuling, Sibley David. 2016. Analysis of noncanonical calcium-dependent protein kinases in Toxoplasma gondii by targeted gene deletion using CRISPR/Cas9. Infect Immun. 84(5):1262-73.

Commentary: Bzik DJ. 2016. Serial Dissection of Parasite Gene Families. Infect Immun. 84(5):1252-4. Doi:10.1128/IAI.00175-16.

10. Kuhlenschmidt TB, Rutaganira FU, Long S, Tang K, Shokat KM, Kuhlenschmidt MS, Sibley LD. 2015. Inhibition of calcium-dependent protein kinase 1 (CDPK1) in vitro pyrazolopyrimidine derivatives does not correlate with sensitivity of Cryptosporidium parvum growth in cell culture. Antimicrob Agents Chemother. 60(1):570-9.

11. Piya C, Horáková E, Long S, Černotíková-Stříbrná E, Lindsay M. Esteban JM. Bontempi, Lukeš J. 2013. Both human ferredoxins equally efficiently rescue ferredoxin deficiency in trypanosomes. Mol. Microbiol. 89: 135-51. 

12. Anastasios D. Tsaousis, Sandrine Ollagnier-de Choudens, Eleni Gentekaki, Long S, Daniel Gaston, Alexandra Stechmann, Daniel Vinella, Beatrice Py, Marc Fontecave, Frederic Barras, Julius Lukeš and Andrew J. Roger. 2012. The evolution of Fe-S cluster biogenesis in the anaerobic parasite Blastocystis. Proc. Natl. Acad. Sci. USA. 109: 10426-10431.

13. Long S, Piya C, Tsaousis DA, Skalicky T, Verner Z, Wen YZ, Roger AJ, Lukeš J. 2011. Stage-specific essentiality of Isa1 and Isa2, mitochondrial Fe/S cluster assembly proteins in the excavate protist Trypanosoma brucei. Mol. Microbiol. 81: 1403-1418.

14. Týč J, Long S, Jirků M, Lukeš J. 2010. YCF45 protein, usually associated with plastids, is targeted into the mitochondrion of Trypanosoma brucei. Mol. Biochem. Parasitol. 173: 43-47

15. Wohlgamuth-Benedum JM, Rubio MT, Paris Z, Long S, Poliak P, Lukeš J, Alfonzo JD. 2009. Thiolation controls cytoplasmic tRNA stability and acts as a negative determinant for tRNA editing in mitochondria. J. Biol. Chem. 284: 23947-23953.

16. Long S, Jirků M, Ayala FJ, Lukeš J. 2008. Mitochondrial localization of human frataxin is necessary but processing is not for rescuing frataxin deficiency in Trypanosoma brucei. Proc. Natl. Acad. Sci. USA. 105: 13468-13473.

17. Long S, Vavrova Z, Lukeš J. 2008. The import and function of diatom and plant frataxins in the mitochondrion of Trypanosoma brucei. Mol. Biochem. Parasitol. 162: 100-104. IF=2.035

18. Long S, Jirků M, Mach J, Ginger ML, Sutak R, Des Richardson R, Tachezy J, Lukeš J. 2008. Ancestral roles of eukaryotic frataxin: mitochondrial frataxin function and heterologous expression of hydrogenosomal Trichomonas homologues in trypanosomes. Mol. Microbiol. 69: 94-109.

 

 





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