报告人：郭琳琳 博士

时间：2016年5月9日（周一）14:20

地点：动医动科大楼418。

联系人：潘保良 baoliang@cau.edu.cn

 

郭琳琳简历

Education

PhD, Biomedical Genetics, Genomics and Development (GGD) program，University of Rochester Medical Center (URMC), Rochester, NY                                                      March 2016                                          

Master of Science (MS), University of Rochester, School of Medicine and Dentistry              June 2013                                  

Bachelor Degree, China Agricultural University (CAU), Beijing, China                          June 2010                                                        

 

Research & development Experience 

PhD candidate, Heinrich Jasper lab, Genetics Program, URMC                     June 2011- Present                                                        

Oncology Biomarker Development (OBD), gRED, Genentech, Inc, South San Francisco.  Oct 2014 - Oct 2015          

Co-op (Intern), Alex Huang Lab  Measuring pathway modulation in the clinic: mitigating operational limitations with ABCD  biomarker assay platform  in collaboration with Nanostring  Technologies, Inc.

n  The Validation of a novel protein profiling platform that uses Abs coupled with DNA - barcoded sensing (ABCD) technology to measure total or phospho - protein levels.   This incorporates the optimization and writing of the protocols and lab manuals for this new assay.   

n  Compare this new biomarker assay platform with other signal detection platforms such as: RPPA, WB, FACS, etc on cell lines and clinical samples   outsourcing through Theranostics Health specialized in RPPA.   

n  Test the ABCD platform in cancer cell lines in a multiplex setting, on the low/single cell number level.

n  Examine the new technology in clinical samples: Fine Needle Aspirates (FNAs) or Circulating Tumor Cells (CTCs) in multiplex Abs and on low/single cell number resolution. This involves the interaction for clinical protocol review and optimization with external service company Fluxion, Inc which specializes in single cell isolation and clinical samples procurement.  

n  Collaborate with biomarker bioinformatics team in Genentech and Nanostring on data analysis. Present project data in project team meetings, scientific researcher meetings and department seminars. 

n  Related training experience: FACS, assay development, cell culture and treatments (cell - based ligand binding assays), signal amplification, western blot, RPPA protein sample preparation, blood/plasma/patient samples handling.

Graduate student, Buck Institute for Research on Aging, Novato, CA                July 2012 - Oct 2014                                       

(lab relocated from NY to CA)                                                

Investigated intestinal immune homeostasis and lifespan in Drosophila – findings could be useful to develop therapies for disorders like inflammatory bowel disease (IBD), allergic and autoimmune diseases, obesity, diabetes as well as cancer and other chronic inflammatory diseases. The following specific areas were studied:

•   The relationship between dysbiosis and dysplasia in the aging fly intestine.

•   How Foxo (Forkhead box O) causes  age- related immunosenescence . 

•   Peptidoglycan Recognition Protein SC class (PGRP- SC2) promotes intestinal homeostasis and extends lifespan.  

   Used Illumina sequencing technology to examine intestinal microbe composition changes in young and aging flies. Familiar with 16S Metagenomic Sequencing Library Preparation, Miseqrun and data interpretation.

   Investigated the association between dysplasia, dysbiosis and genotype s using GWAS.  Identified single nucleotide peptides (SNPs) in term of their effects on intestinal stem cell proliferation and bacteria number. 

   Analyzed the interaction between Foxo, endoplasmic reticulum (ER) stress, intestinal bacteria, and intestinal stem cell proliferation.