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姓名：王晓佳 |
性别：
女 |
职务： |
| 所在系：
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职称：副教授 |
导师类别：博士 |
| 政治面貌：中共党员 |
出生年月：1976.7 |
学位：博士 |
| 电子邮件：wangxj@cau.edu.cn |
办公电话：62734377 |
| 个人简介：个人简介：
2006年12月受聘为中国农业大学动物医学院副教授，研究方向为重要畜禽病毒性疾病的致病机制与新型防控技术。作为第一主讲人，负责全校研究生选修课《蛋白质及蛋白质技术》，第三主讲国家级精品课程《兽医寄生虫学》。2010年3月-2011年9月受聘为美国芝加哥大学微生物系访问助理教授，研究方向为疱疹病毒与宿主细胞的相互作用机理。
2006年“副粘病毒入侵宿主细胞的分子机制研究”获得教育部全国“百篇”优秀博士论文。近年以第一作者或通讯作者在国际权威的PNAS（美国科学院报）、Virology（病毒学杂志）等SCI收录期刊发表论文11篇，国内核心杂志发表论文4篇，申请发明专利4项，参编著作4部。主持高等学校全国优秀博士学位论文作者专项资金、国家自然科学基金、国家十一五攻关、教育部博士点新教师基金、国家十五攻关、科技部基本科研业务费各1项，副主持国家高技术研究发展计划（863）1项。
熟练掌握英语与俄语，积极推进国际合作。2008年获德意志学术中心邀请与资助，在德国汉诺威大学医学院展开3个月的国际合作研究，并受邀报告；2008年在教育部的外教师资计划资助下，邀请美国农业部的知名专家，在校内组织开展一系列学术讲座。
SCI收录论文（* 通讯作者）：
Wang, X.J., Patenode, C., Roizman, B. The US3 protein kinase of HSV-1 cycles between the cytoplasm and nucleus and interacts with PDCD4 to block apoptosis. Proc Natl Acad Sci USA, 2011, 108 (35), 14632-36.
Wang, X.J., * Li, C.G., Chi, X.J., Wang, M. Characterisation and evaluation of antiviral recombinant peptides based on the heptad repeat regions of NDV and IBV fusion glycoproteins. Virology, 2011, 416 (1-2), 65-74.
Wang, X.J. * Chi, X.J., Wang, M. Structural characteristics and antiviral activity of multiple peptides derived from MDV glycoproteins B and H. Virology Journal, 2011, 8, 190-203.
Wang, X.J. * Heat-shock protein 70 is associated with the entry of Marek’s disease virus into cells. Acta Virologica, 2011, 55, 3.
Wang, X.J. * The molecular mechanism of herpesvirus membrane fusion, Progress in Biochemistry and Biophysics, 2010, 37 (6), 583-88. (review article)
Wang, X.J. and Wang, M. The molecular mechanism of class II enveloped viruses membrane fusion, Progress in Biochemistry and Biophysics, 2007, 34 (7), 682-86. (review article)
Wang, X.J., Bai, Y.D., Zhang, G.Z., Zhao, J.X., Wang, M., and Gao, G.F. Structure and function study of paramyxovirus fusion protein heptad repeat peptides, Archives of Biochemistry and Biophysics, 2005, 436 (2), 316-322.
Wang, X.J., Zhang, G.Z., Zhao, J.X., and Wang, M. Interacting domains of the HN and F proteins of paramyxovirus, Chinese Science Bulletin, 2005, 50 (20), 2231-2234.
Wang, X.J., Zhu, D.B., Zhang, G.Z., Bai ,Y.D., and Wang, M. Construction and expression of correlative genes with membrane fusion of paramyxovirus, Progress in Biochemistry and Biophysics, 2005, 32 (3), 228-34.
Wang, X.J., Xu, Y.H., Cole ,D.K., Lou, Z.Y., Liu, Y.W., Rao, Z.H., Wang, M., and Gao, G.F. Biochemical, biophysical and X-ray crystallographic analyses of the fusion core of Sendai virus F protein, Acta Crystallographica Section D, 2004, 60 (9), 1632-1635.
Wang, X.J., Zhang, W.H., Wang, M., and Gao, G.F. The Molecular mechanism of enveloped virus-cell membrane fusion, Progress in Biochemistry and Biophysics, 2004, 31 (6), 482-491. (review article)
专利：
1. HR2模拟蛋白及其编码基因与应用. 200610144153.（第1作者）
2. Novel application of Coccidian. 200710064924（第2作者）
3. 鸡球虫抗药性检测方法及其检测试剂盒. 200910080180（第2作者）
4. 具有抑制病毒混合感染活性的多肽.2010101218301.（第1作者）
负责课题：
1. 马立克氏病毒与宿主细胞膜融合的分子机制与抗病毒抑制研究，高等学校全国优秀博士学位论文作者专项资金，教育部，200769，2007-2012，主持
2. 球虫抗药性检测与控制关键技术研究，2006BAK02A19-4，国家十一五科技支撑计划，科技部，2006-2008，主持
3. HR模拟蛋白的构建、结构与抗病毒功能研究，20070019068，博士点新教师基金，教育部，2008-2010，主持
4. 基于七肽重复区域设计的马立克氏病毒新型抑制物研究，2009JS10，教育部基本科研业务费, 2010年，主持
5. 鸡球虫顶膜抗原（AMA）的细胞入侵作用和免疫原性研究，GSKJ070202，广东省兽医公共卫生公共实验室开放基金项目，广东省科技部，2007-2009，主持
6. 表达并运送禽流感病毒抗原组的新型疫苗活载体研制，2006AA02Z458，科技部“863”计划，科技部，2006-2008，副主持
7. 抑制新城疫与传染性支气管炎病毒混合感染的多肽作用机理，31101819，国家自然基金委，2011-2014，主持
MAJOR RESEARCH EXPERIENCE：
2010-present, This project concerns the herpes simplex virus 1 (HSV-1) US3 and a potential ant-apoptotic target of the enzyme. The US3 protein kinase of herpes simplex virus 1 plays a key role in blocking apoptosis induced by viral gene products or exogenous agents. We find that in the yeast two hybrid system a domain of US3 essential for anti-apoptotic activity reacted with PDCD4 (Programmed Death protein 4). US3 interacts with PDCD4, and PDCD4 is posttranslationally modified in infected cells both in a US3 dependent and independent fashion and that depletion of PDCD4 by siRNA blocked apoptosis induced by mutant virus. In infected cells PDCD4 accumulates in the nucleus whereas US3 accumulates in the cytoplasm. Studies designed to elucidate the convergence of these proteins led to the discovery that US3 protein kinase cycles between the nucleus and cytoplasm and that US3 retains PDCD4 in infected cell nuclei. Part of the result was published in Proc Natl Acad Sci USA.
2008-present, Losses caused by mixed virus infections are much more devastating than single virus infections. Newcastle disease virus (NDV) and infectious bronchitis virus (IBV), serious threats to the poultry industry, can give rise to complex mix-infections which hinder diagnosis and prevention. In this study, it is shown that the newly designed peptides, which are developed based on the heptad repeat-2 (HR2) region of the fusion glycoproteins from NDV and IBV, can show a more potent antiviral activity than the mother HR2 peptides. Experiments conducted on the plaque formation with chicken embryo assays confirmed the result. The designed peptides can completely inhibit single virus infections and mixed infection caused by NDV and IBV. Further, cell toxicity and possible targets for peptides have been studied, thereby strengthening the notion that HR2 is an attractive site for therapeutic intervention. The present paper offers the possibility of designing a relatively broad-spectrum class of antiviral mix-infection inhibitors. A Chinese patent has been awarded. Part of the result was published in Virology.
2008-present, Study on Interactions between Envelope Proteins and their Cellular Receptors of Marek′s Disease Virus. Results from a Western blot analysis of cellular proteins for virus receptors and co-immunoprecipitation suggests that heat shock protein 70 (HSP70) is a potential cellular receptor for MDV glycoprotein gH. Plaque inhibition assays confirm the involvement of HSP70 in the early stages of MDV entry into chicken embryo fibroblasts (CEF). The present work supports that HSP70 is implicated in the MDV entry process by binding to gH, and enhances the understanding of multifunctional HSP70 and the MDV infection process. Part of the result was published in Acta Virologica. Another paper is being prepared for publication.
2007-present, Study on functional domains analysis of MDV gH and gB, to determine the peptides homolog region in which virus entry can be inhibited. Novel heptad repeat (HR) peptides, modified by N-4EK/C-4K, with cascade connection of antiviral HR regions from different stages during virus entry, were shown to be more potent antiviral inhibitors. CD and Gel-filtration and IP were used to find inhibitor’s target. The animal experiments further proved that the novel construction peptides provided a stable and highly effective strategy for developing novel antiviral drugs. A Chinese patent has been awarded. Part of the result was published in Virology Journal.
2004-2006, Study on HN and F Glycoprotein Interaction of Paramyxovirus. The GST-pull down and MS and CD results showed that the globular head domain of HN could interact with HR1 and HR2 of F protein. The studies provided direct insight into the interaction domain of the two glycoproteins of paramyxovirus, and consequently completed the molecular mechanism in which the HN protein activates the F protein inducing membrane fusion. Results were published in Chinese Science Bulletin and Progress in Biochemistry and Biophysics.
2001-present, Ph.D thesis: Molecular Mechanism of Paramyxovirus Entering Host Cell. The CD and Gel-filtration and crystal diffracting X-rays results showed that heptad repeat (HR) regions of HR1 and HR2 from the F glycoprotein of paramyxoviruses form 6-helix bundle (trimer-of-hairpins), the post-fusion conformation of the fusion core of the paramyxovirus. The virus-cell experiment showed only soluble HR2 peptide had potent and specific virus-cell fusion inhibition activity. The outcome provided a solid basis for developing novel antiviral drugs and recruiting a new theory in virology. Results were published in Acta Crystallographica Section D, Archives of Biochemistry and Biophysics, and Progress in Biochemistry and Biophysics. |
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